Human Mesothelin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB32651
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 420404
Product Summary for Human Mesothelin Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272
Glu296-Gly580
Accession # AAH09272
Specificity
Detects human Mesothelin in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Mesothelin Antibody
Mesothelin in Human Ovarian Cancer Tissue.
Mesothelin was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 5 µg/mL Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32651) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Mesothelin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB32651 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mesothelin
Alternate Names
CAK1, MPF, MSLN, SMR
Gene Symbol
MSLN
UniProt
Additional Mesothelin Products
Product Documents for Human Mesothelin Antibody
Product Specific Notices for Human Mesothelin Antibody
For research use only
Loading...
Loading...
Loading...
Loading...