Human Mesothelin Propeptide/MPF Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3448

R&D Systems, part of Bio-Techne
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AF3448
AF3448-SP
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Biotin
BAF3448
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Mesothelin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin Propeptide/MPF isoform 1
Arg35-Leu289
Accession # Q13421

Specificity

Detects human Mesothelin Propeptide/MPF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with mature recombinant human Mesothelin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Mesothelin Propeptide/MPF Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Mesothelin Propeptide/MPF
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin is a 40 kDa, 292 amino acid, GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic pro-region that is removed when a GPI-anchor is added. The amino‑terminal propeptide is cleaved by furin to release a33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). Mouse MPF has been shown to potentiate megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. The amino acid sequence of human MPF is 55% identical to that of mouse or rat MPF.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

Q13421

Additional Mesothelin Products

Product Documents for Human Mesothelin Propeptide/MPF Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin Propeptide/MPF Antibody

For research use only

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