Human MFAP3 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB113251U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 1059143
Product Specifications
Immunogen
Human embryonic kidney cell, HEK293-derived human MFAP3L
Met1-Met149
Accession # O75121
Met1-Met149
Accession # O75121
Specificity
Detects human MFAP3L in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human MFAP3 Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Titration recommended for
optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample
used for this experiment was HEK293 cells transfected with hMFAP-3L and eGFP.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2
mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MFAP3
Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non‐fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly (1, 2). In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions (1, 2). Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other (1, 2). Human MFAP3L shows 71% amino acid (aa) sequence homology to MFAP3, but not other MFAPs (3). Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif (3). The ECD of human MFAP3L shares 89% and 90% aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin (1, 2). MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression (1, 2). MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation (3). Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression (3).
References
- Zhu, S. et al. (2020) J Cell Physio. 236:41.
- Mecham, R.P. et al. (2015) Matrix Biol. 47:13.
- Lou, X. et al. (2014) BBA. 1842:1423.
Long Name
Microfibrillar-Associated Protein 3
Alternate Names
DKFZp586F0219, microfibril-associated glycoprotein 3, microfibrillar-associated protein 3
Gene Symbol
MFAP3
UniProt
Additional MFAP3 Products
Product Specific Notices for Human MFAP3 Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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