HEK293 human embryonic kidney cell line transfected with (A) human MGLUR1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MGLUR1 Monoclonal Antibody (Catalog # MAB48361) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human mGluR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human MGLUR1 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR1

Metabotropic glutamate receptor 1 (mGluR1 alpha) is a 130 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. On neurons, mGluR1 is postsynaptic, associates with G_q-like proteins, mobilizes intracellular Ca⁺⁺, and influences ion channel activity. Mature mGluR1 is 1176 amino acids (aa) in length and contains a 574 aa N-terminal extracellular domain (ECD) (aa 19‑592). The ECD binds glutamate and forms either a covalent homodimer, or heterodimer with CaSR. There is one alternative splice form for human mGluR1 that shows a 20 aa substitution for the C-terminal 308 amino acids. Over aa 33‑522, human mGluR1 shares more that 98% aa identity with mouse, rat and canine mGluR1.

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14816 (Mouse); 24414 (Rat)

Gene Symbol

GRM1

UniProt

Q13255

Additional mGluR1 Products

Product Documents for Human mGluR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human mGluR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human mGluR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB48361

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all mGluR1 Antibodies »

Product Summary for Human mGluR1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human mGluR1 Antibody

Detection of MGLUR1 in HEK293 Human Cell Line Transfected with Human MGLUR1 and eGFP by Flow Cytometry.