HEK293 human embryonic kidney cell line transfected with (A) human mGluR3 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human mGluR3 Monoclonal Antibody (Catalog # MAB46682) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

mGluR3 in Human Brain.

mGluR3 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human mGluR3 Monoclonal Antibody (Catalog # MAB46682) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human mGluR3 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human mGluR3 and eGFP

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR3

Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2.

Long Name

Metabotropic Glutamate Receptor 3

Alternate Names

GPRC1C, GRM3

Entrez Gene IDs

2913 (Human); 108069 (Mouse); 24416 (Rat)

Gene Symbol

GRM3

UniProt

Q14832

Additional mGluR3 Products

Product Documents for Human mGluR3 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human mGluR3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human mGluR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB46682

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all mGluR3 Antibodies »

Product Summary for Human mGluR3 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human mGluR3 Antibody

Detection of mGluR3 in HEK293 Human Cell Line Transfected with Human mGluR3 and eGFP by Flow Cytometry