Human MIA Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB20501
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 294203
Product Specifications
Immunogen
E. coli-derived recombinant human MIA
Gly25-Gln131
Accession # Q16674
Gly25-Gln131
Accession # Q16674
Specificity
Detects human MIA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 or recombinant rat IL-5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MIA Antibody
MIA in Human Pancreas.
MIA was detected in immersion fixed paraffin-embedded sections of human pancreas array using Mouse Anti-Human MIA Monoclonal Antibody (Catalog # MAB20501) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MIA Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of normal human pancreas
Sample: Immersion fixed paraffin-embedded sections of normal human pancreas
Reviewed Applications
Read 1 review rated 4 using MAB20501 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIA
Long Name
Melanoma Inhibitory Activity
Alternate Names
CD-RAP
Gene Symbol
MIA
UniProt
Additional MIA Products
Product Documents for Human MIA Antibody
Product Specific Notices for Human MIA Antibody
For research use only
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