Human Mineralocorticoid R/NR3C2 Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-H3122-00

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
PP-H3122-00
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Direct ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H3122

View all Mineralocorticoid R/NR3C2 Antibodies »

Product Summary for Human Mineralocorticoid R/NR3C2 Antibody

Immunogen

Recombinant human Mineralocorticoid R/NR3C2
aa 2-99

Specificity

This antibody specifically recognizes human MR and cross-reacts with mouse and rat MR. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Mineralocorticoid R/NR3C2 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect human MR.

Immunohistochemistry

This antibody can be used at 20 - 40 μg/mL with the appropriate secondary reagents to detect human MR.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human MR.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Mineralocorticoid R/NR3C2

Mineralcorticoid Receptor (MR; NR3C2) is a member of the Steroid Receptor superfamily (AR,ER,GR,PR). Aldosterone binds to MR with a high affinity. MR is expressed in the kidney, gut, brain, pituitary, hypothalamus and heart. MR plays a critical role in regulating electrolyte and fluid balance in the kidney, as well as having specific roles in the central nervous system. The protein is expressed as a 107 kDa nuclear form. MR and GR can cooperatively form heterodimers.

Long Name

Mineralocorticoid Receptor

Alternate Names

MineralocorticoidR, MLR, NR3C2

Entrez Gene IDs

4306 (Human)

Gene Symbol

NR3C2

Additional Mineralocorticoid R/NR3C2 Products

Product Documents for Human Mineralocorticoid R/NR3C2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Mineralocorticoid R/NR3C2 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.