Human MKK4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3390
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 323042
Product Summary for Human MKK4 Antibody
Immunogen
E. coli-derived recombinant human MKK4
Met1-Asp399
Accession # P45985
Met1-Asp399
Accession # P45985
Specificity
Detects human MKK4 in direct ELISAs. This antibody does not cross-react with recombinant human MKK7.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MKK4 Antibody
MKK4 in HeLa Human Cell Line.
MKK4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human MKK4 Monoclonal Antibody (Catalog # MAB3390) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human MKK4 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 3.8 using MAB3390 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKK4
Mitogen-activated protein kinase kinase 4 (MKK4 or MAP2K4), also known as SAPK/ERK kinase 1 (SEK1) and Jun kinase kinase 1 (JNKK1), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser257 and Thr261 by several upstream MAPK kinase kinases (MAP3Ks). MKK4 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.
Long Name
Mitogen-activated Protein Kinase Kinase 4
Alternate Names
JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1
Entrez Gene IDs
6416 (Human)
Gene Symbol
MAP2K4
UniProt
Additional MKK4 Products
Product Documents for Human MKK4 Antibody
Product Specific Notices for Human MKK4 Antibody
For research use only
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