Human MLK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5066
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MLK2
His345-Arg495
Accession # Q02779
His345-Arg495
Accession # Q02779
Specificity
Detects endogenous human MLK2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human MLK2 Antibody
Detection of Human MLK2 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK2 at approximately 104 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human MLK2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line
Sample: Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MLK2
MLK2 (mixed lineage kinase 2; also MST and MAP3K10) is a member of the STE Ser/Thr kinase family of enzymes. It is expressed in neurons, lymphoid tissue, and skeletal muscle, where it participates in apoptosis. Human MLK2 is 954 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 23‑76), followed by a kinase domain that likely acts to phosphorylate Ser/Thr (aa 99‑355), two Leu-zipper segments that mediate homodimerization (aa 384‑419) and a C-terminal Ser/Thr/Pro-rich region that undergoes phosphorylation at multiple sites (aa 497‑954). There is one splice variant that shows an in-frame 16 aa substitution for the 16 aa between aa 465‑480.
Long Name
Mixed Lineage Kinase 2
Alternate Names
MAP3K10, MST
Gene Symbol
MAP3K10
UniProt
Additional MLK2 Products
Product Documents for Human MLK2 Antibody
Product Specific Notices for Human MLK2 Antibody
For research use only
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