Human MMP-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF910
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-10 (R&D Systems, Catalog # 910-MP)
Tyr18-Cys476
Accession # P09238
Tyr18-Cys476
Accession # P09238
Specificity
Detects human MMP-10 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP‑3 and rhMMP‑7 is observed under non-reducing conditions. Approximately 10% and 2% cross-reactivity with rhMMP-3 and rhMMP-2 is observed under reducing conditions, respectively. Additionally, less than 1% cross-reactivity with rhMMP-1, -8, -9, -12, -13, and recombinant mouse MMP-9 is observed under reducing and non-reducing conditions.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MMP-10 Antibody
MMP-10 in Human Placenta.
MMP-10 was detected in immersion fixed paraffin-embedded sections of human placenta using Human MMP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF910) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MMP-10 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus) and placenta
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus) and placenta
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using AF910 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-10
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short linker region, and a carboxyl terminal hemopexin-like domain.
Long Name
Matrix Metalloproteinase 10
Alternate Names
MMP10, Stromelysin 2
Entrez Gene IDs
4319 (Human)
Gene Symbol
MMP10
UniProt
Additional MMP-10 Products
Product Documents for Human MMP-10 Antibody
Product Specific Notices for Human MMP-10 Antibody
For research use only
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