Human MMP-7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9074
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1036221
Product Summary for Human MMP-7 Antibody
Immunogen
Mouse myeloma cell line NS0-derived human MMP-7
Leu18-Lys267
Accession # P09237
Leu18-Lys267
Accession # P09237
Specificity
Detects human MMP-7 in sandwich ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MMP-7 Antibody
MMP-7 in Capan-1 Human Cell Line.
MMP-7 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line (positive staining) and HeLa human cervical epithelial carcinoma cell line (negative control) using Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9074) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007)) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human MMP-7 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MMP‑7 Monoclonal Antibody (Catalog # MAB9073).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Capan‑1 human pancreatic adenocarcinoma cell line
Sample: Immersion fixed Capan‑1 human pancreatic adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-7
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.
Long Name
Matrix Metalloproteinase 7
Alternate Names
Matrilysin, MMP7
Gene Symbol
MMP7
UniProt
Additional MMP-7 Products
Product Documents for Human MMP-7 Antibody
Product Specific Notices for Human MMP-7 Antibody
For research use only
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