Human MMP-7 Antibody

Detection of Human MMP‑7 by Western Blot.

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human MMP‑7 by Simple Western^TM.

Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, human small intestine tissue, and CaSki human cervical carcinoma, loaded at 0.5 mg/mL. Specific bands were detected for MMP‑7 at approximately 49 and 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human MMP-7 by Western Blot

Zymography analysis for active MMP2 was performed with 4 μg of total protein of the conditioned medium of U87 (a) and primary IC cells (b) after 48 h exposure to JS-K (1–3 μM) on gelatin-containing PAGE. Western blot analysis for MMP2, MMP7, MMP9 and TIMP3 was performed for conditioned medium of U87 (a) and IC (b) cells with 4 μg of total protein as well as of 20 μg of total cell lysate of U87 (c) and IC cells (d) on SDS-PAGE. Conditioned media of knockdown and overexpression of ATF3 were examined for active MMP2 as well as the plasmid controls and uninfected U87 (e). 4 μg of protein of the supernatant (e) and 20 μg of cell lysates (f) were screened for MMP2, MMP7, MMP9 and TIMP3 by SDS-PAGE. Coomassie staining of the gels was used as a loading control for zymogram and for western blot of the supernatant, GAPDH was used as a loading control for cell lysates. Figures are representative for three independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28250971), licensed under a CC-BY license. Not internally tested by R&D Systems.