Human MMP-9 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF911
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rabbit
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
ELISA Microarray Development, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay (PLA), Western Blot, Zymography
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Specificity
Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MMP-9 Antibody
MMP-9 in NS0 Mouse Cell Line.
MMP-9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MMP‑9 in Human Ovarian Cancer Tissue.
MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP‑9 in MCF‑7 Human Cell Line.
MMP-9 was detected in immersion fixed MCF-7 human breast cancer cell line using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human MMP-9 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 and immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 and immersion fixed MCF-7 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-9 (Catalog # 911-MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-9 (Catalog # 911-MP), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human MMP-9 Western Blot Standard (Catalog # WBC018)
Sample: Recombinant Human MMP-9 Western Blot Standard (Catalog # WBC018)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.2 using AF911 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules.
Long Name
Matrix Metalloproteinase 9
Alternate Names
CLG4B, Gelatinase B, GELB, MANDP2, MMP9
Gene Symbol
MMP9
Additional MMP-9 Products
Product Documents for Human MMP-9 Antibody
Product Specific Notices for Human MMP-9 Antibody
For research use only
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