Human/Mouse Aurora B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4006
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Aurora B
Met1-Ile150
Accession # Q96GD4
Met1-Ile150
Accession # Q96GD4
Specificity
Detects human and mouse Aurora B in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Aurora B Antibody
Detection of Human Aurora B by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Aurora B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora B at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse Aurora B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora B
Aurora B, a serine/threonine protein kinase, is a key regulator of the onset of cytokinesis and chromosome segregation. Phosphorylation of Histone H3 at Ser 10 and Ser 28 by Aurora B contributes to chromosome number instability and mitotic chromosome condensation. In addition, Aurora B phosphorylates MCAK (mitotic centromere-associated kinesin) on Ser 92 and this inhibits MCAK’s microtubule depolymerizing activity. Aurora B localizes to kinetochore fibers during chromosome movement and segregation.
Alternate Names
Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5
Gene Symbol
AURKB
UniProt
Additional Aurora B Products
Product Documents for Human/Mouse Aurora B Antibody
Product Specific Notices for Human/Mouse Aurora B Antibody
For research use only
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