Human/Mouse/Bovine Integrin alpha6/CD49f APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB13501A
Clone GoH3 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Human, Mouse, Bovine
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # GoH3
Product Specifications
Immunogen
Mouse mammary tumor cells
Specificity
Detects human, mouse, and bovine Integrin alpha6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha6 subunit.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images
Detection of Integrin alpha6/CD49f in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f APC-conjugated Monoclonal Antibody (Catalog # FAB13501A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin alpha6/CD49f in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f APC-conjugated Monoclonal Antibody (Catalog # FAB13501A) and Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes and mouse splenocytes
Sample: Human peripheral blood lymphocytes and mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 6/CD49f
Integrin alpha6 (also VLA-6, CD49F and ITA6) is a 130-150 kDa member of the Integrin alpha chain family of molecules. Mature Integrin alpha6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail. The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24-938), and a 30-31 kDa membrane-bound light chain (aa 942-1130). Integrin alpha6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin.
Alternate Names
CD49f, ITGA6, Platelet gpl, VLA-6
Gene Symbol
ITGA6
Additional Integrin alpha 6/CD49f Products
Product Specific Notices
For research use only
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