Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CD44 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse CD44 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cell (PBMCs) and mouse splenocytes

Reviewed Applications

Read 1 review rated 5 using FAB6127P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD44

CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

Additional CD44 Products

Product Documents for Human/Mouse CD44 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse CD44 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse CD44 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6127P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD44 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse CD44 PE-conjugated Antibody

Detection of CD44 in Human PBMCs by Flow Cytometry.