Human/Mouse/Chicken RGM-A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2458
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Chicken
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 275407
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-A
Cys48-Gly421
Accession # Q6PCX7
Cys48-Gly421
Accession # Q6PCX7
Specificity
Detects mouse RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human RGM-A and recombinant chicken RGM is observed. In Western blots, approximately 25% cross-reactivity with recombinant mouse RGM-C is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human/Mouse/Chicken RGM-A Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Repulsive Guidance Molecule A/RGM-A (Catalog # 2459-RM)
Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)
Sample: Recombinant Human Repulsive Guidance Molecule A/RGM-A (Catalog # 2459-RM)
Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RGM-A
Long Name
Repulsive Guidance Molecule A
Alternate Names
RGMA
Gene Symbol
RGMA
UniProt
Additional RGM-A Products
Product Documents for Human/Mouse/Chicken RGM-A Antibody
Product Specific Notices for Human/Mouse/Chicken RGM-A Antibody
For research use only
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