Human/Mouse CRY1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3764
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CRY1
Lys115-His224
Accession # Q16526
Lys115-His224
Accession # Q16526
Specificity
Detects human and mouse CRY1 in direct ELISAs and Western Blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse CRY1 Antibody
CRY1 in MOLT‑4 Human Cell Line.
CRY1 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human/Mouse CRY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3764) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse CRY1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testis
Sample: Perfusion fixed frozen sections of mouse testis
Western Blot
0.1 µg/mL
Sample: Recombinant Human CRY1
Recombinant Mouse CRY1
Sample: Recombinant Human CRY1
Recombinant Mouse CRY1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRY1
Long Name
Cryptochrome 1
Alternate Names
Cryptochrome I, PHLL1
Gene Symbol
CRY1
UniProt
Additional CRY1 Products
Product Documents for Human/Mouse CRY1 Antibody
Product Specific Notices for Human/Mouse CRY1 Antibody
For research use only
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