Human/Mouse CXCL12/SDF-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB310
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat, Canine, Transgenic Mouse
Applications
Validated:
Neutralization
Cited:
Bioassay, ELISA Capture, ELISA Development (Capture), Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 79014
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human and mouse CXCL12/SDF‑1 alpha and human CXCL12/SDF‑1 beta.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Mouse CXCL12/SDF-1 Antibody
Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutral-ization by Human/Mouse CXCL12/SDF‑1 Antibody.
Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB310). At 111 µg/mL, this antibody will neutralize > 50% of the chemotactic effect.
Detection of Mouse CXCL12/SDF-1 by In vivo assay
Enumeration of bone marrow and blood Lin−/Sca1+ cell numbers following stroke and SDF1-A Antibody.(A) Twenty-four hour post stroke+SDF1-A antibody mice show a marked increase in the number of Lin−/Sca1+ cells present in the bone marrow compared to controls. (B) Twenty-four hour post stroke mice showed a marked decrease in the mobilization of these cells to the blood. * P<0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24465621), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CXCL12/SDF-1 by In vivo assay
Administration of Lin−/Sca1+ cells post stroke.A. Administration of Lin−/Sca1+ cells post stroke significantly reduced infarct volume at 24 hours post stroke (*P<0.05). B. Administration of the Lin−/Sca1+ cells with an SDF1-A antibody (AB) no longer demonstrates a beneficial reduction in the infarct volume (p = NS). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24465621), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse CXCL12/SDF-1 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CXCL12/SDF‑1 alpha-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. At 111 μg/mL, this antibody will neutralize > 50% of the chemotactic effect due to 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL12/SDF-1
CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.
Alternate Names
PBSF, SDF-1, SDF1
Gene Symbol
CXCL12
UniProt
Additional CXCL12/SDF-1 Products
Product Documents for Human/Mouse CXCL12/SDF-1 Antibody
Product Specific Notices for Human/Mouse CXCL12/SDF-1 Antibody
For research use only
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