Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC350F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 79018
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL12/SDF-1 alpha
Lys22-Lys89
Accession # Q6ICW0
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCL12/SDF-1
CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.
Alternate Names
PBSF, SDF-1, SDF1
Gene Symbol
CXCL12
UniProt
Additional CXCL12/SDF-1 Products
Product Documents for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
Product Specific Notices for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...