Human/Mouse DAPP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7024

R&D Systems, part of Bio-Techne
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AF7024
AF7024-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all DAPP1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DAPP1
Gly2-Ser163
Accession # Q9UN19

Specificity

Detects human and mouse DAPP1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse DAPP1 Antibody

Detection of Human and Mouse DAPP1 antibody by Western Blot.

Detection of Human and Mouse DAPP1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DAPP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7024) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse DAPP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAPP1

DAPP1 (Dual Adaptor for Phosphotyrosine and 3-Phosphoinosidides 1; also Bam32 and PHISH) is a 31-32 kDa member of Ig-superfamily of proteins. It shows restricted expression, being found in mast cells, dendritic cells, and germinal center B cells. With respect to B cells, and upon B cell receptor engagement, PI3-kinase is activated, resulting in the generation of membrane-embedded PI(3,4)P2. This product serves as a ligand for cytosolic DAPP1, resulting in its immobilization at the cell membrane. Here, it is phosphorylated on Tyr139, directly regulating HPK1 (hematopoietic progenitor kinase 1) activity, and indirectly regulation HPK1 downstream targets ERK and JNK. Functionally, DAPP1 plays a role in BCR internalization, antibody isotype switching, antigen processing and presentation, and B cell survival. Human DAPP1 is 280 amino acids (aa) in length. It contains one SH2 domain (aa 35‑129), a utilized phosphorylation site at Tyr139 and a C-terminal PH domain (aa 164‑259). When phosphorylated, the observed MW of DAPP1 may be increased by 2-4 kDa in SDS-PAGE. There are four potential alternative splice variants. Two contain a five and 22 aa substitution for aa 259-280, respectively, while a third possesses a 14 aa substitution for aa 1-229, and a fourth shows deletions of aa 35‑75 and aa 180-200 coupled to a three aa substitution for aa 249-280. Over aa 1-163, human DAPP1 shares 91% aa sequence identity with mouse DAPP1.

Long Name

Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1

Alternate Names

BAM32

Entrez Gene IDs

27071 (Human); 26377 (Mouse); 362046 (Rat)

Gene Symbol

DAPP1

UniProt

Q9UN19

Additional DAPP1 Products

Product Documents for Human/Mouse DAPP1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse DAPP1 Antibody

For research use only

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