Human/Mouse Doublecortin/DCX Antibody
R&D Systems, part of Bio-Techne | Catalog # AF10025
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E.coli-derived recombinant human Doublecortin/DCX
Met116-Thr293
Accession # O43602
Met116-Thr293
Accession # O43602
Specificity
Detects human Doublecortin/DCX in direct ELISAs and Western blots. Detects mouse Doublecortin/DCX in immunohistochemistry.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse Doublecortin/DCX Antibody
Detection of Human Doublecortin/DCX by Western Blot.
Western blot shows lysates of human motor cortex tissue, human hypothalamus tissue, and human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doublecortin/DCX at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Doublecortin/DCX in Mouse Brain.
Doublecortin/DCX was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse Doublecortin/DCX Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Western Blot
1 µg/mL
Sample: Human motor cortex tissue, Human hypothalamus tissue, and Human hippocampus tissue
Sample: Human motor cortex tissue, Human hypothalamus tissue, and Human hippocampus tissue
Reviewed Applications
Read 2 reviews rated 4 using AF10025 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Doublecortin/DCX
DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.
Long Name
Doublecortin
Alternate Names
DBCN, Doublecortex, Doublin, Lis-X, LISX
Gene Symbol
DCX
UniProt
Additional Doublecortin/DCX Products
Product Documents for Human/Mouse Doublecortin/DCX Antibody
Product Specific Notices for Human/Mouse Doublecortin/DCX Antibody
For research use only
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