Human/Mouse EBF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7006
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse EBF-2
Arg407-Ser519
Accession # O08792
Arg407-Ser519
Accession # O08792
Specificity
Detects mouse EBF-2 in direct ELISAs. Detects mouse and human EBF-2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse EBF-2 Antibody
Detection of Human and Mouse EBF-2 by Western Blot.
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for EBF-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
EBF-2 in Mouse Embryo.
EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse EBF-2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Western Blot
1 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF7006 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EBF-2
EBF-2 (Early B cell Factor 2; also Mmot1, OLF3 and COE2) is a 62 kDa (predicted) member of the COE family of transcription factors. It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts. In immature osteoblasts, EBF-2 appears to upregulate OPG, suppressing osteoclast formation. And in the developing retina, EBF-2 is found in ganglion, glycinergic Amacrine and horizontal cells, possibly promoting their development over that of photoreceptor cells. Mouse EBF-2 is 575 amino acids (aa) in length. It contains one DNA-binding region with an embedded C5-type zinc‑finger motif (aa 62-238), a dimerization ITP/TIG domain (aa 253-336), and a Pro/Ser-rich transactivation domain (aa 453-534). Although considered an HLH type transcription factor, it does not contain the typical "b", or basic amino acid sequence associated with bHLH factors. EBF-2 both homodimerizes, and heterodimerizes with EBF-1 and -3. There is an alternative start site at Met23. Over aa 407-519, mouse EBF-2 is identical in aa sequence to rat EBF-2 and shares 99% aa identity with human EBF-2.
Long Name
Early B-cell Factor 2
Alternate Names
COE2, EBF2, Mmot1
Gene Symbol
EBF2
UniProt
Additional EBF-2 Products
Product Documents for Human/Mouse EBF-2 Antibody
Product Specific Notices for Human/Mouse EBF-2 Antibody
For research use only
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