Human/Mouse EphB6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3384
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 465323
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EphB6
Val17-Thr579
Accession # O15197
Val17-Thr579
Accession # O15197
Specificity
Detects human and mouse EphB6 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) EphB6 is observed, 25% cross-reactivity with recombinant human (rh) EphA10 is observed, and no cross-reactivity with rhEphA1, 2, 5, rhEphB3, 4, rmEphA1, 3, 4, 6, 7, 8, rmEphB2, 3, recombinant rat (rr) EphA5, or rrEphB1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human/Mouse EphB6 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Recombinant Mouse EphB6 Fc Chimera (Catalog # 611-B6)
Sample: Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Recombinant Mouse EphB6 Fc Chimera (Catalog # 611-B6)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3384 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB6
EphB6, also known as Hep and Mep, is a 110 kDa type I transmembrane glycoprotein in the Eph receptor tyrosine kinase family. EphB6 is expressed in brain, pancreas, thymus, kidney, and on peripheral T cells. Depending on concentration, Ephrin-B2 can either inhibit or promote EphB6 mediated cell adhesion and migration. EphB6 participates in T cell activation, and its level of expression is inversely correlated with tumor aggressiveness in a variety of malignancies. Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6.
Long Name
Eph Receptor B6
Alternate Names
Hep, Mep
Gene Symbol
EPHB6
UniProt
Additional EphB6 Products
Product Documents for Human/Mouse EphB6 Antibody
Product Specific Notices for Human/Mouse EphB6 Antibody
For research use only
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