Human/Mouse FIH-1/HIF-1AN Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7874
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 745122
Product Specifications
Immunogen
E. coli-derived recombinant human FIH-1/HIF-1AN
Met1-Asn349
Accession # Q9NWT6
Met1-Asn349
Accession # Q9NWT6
Specificity
Detects human FIH-1/HIF-1AN in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse FIH-1/HIF-1AN Antibody
Detection of Human and Mouse FIH-1/HIF-1AN by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse FIH-1/ HIF-1AN Monoclonal Antibody (Catalog # MAB7874) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FIH-1/HIF-1AN at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FIH-1/HIF-1AN in A172 Human Cell Line.
FIH-1/HIF-1AN was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human/Mouse FIH-1/HIF-1AN Monoclonal Antibody (Catalog # MAB7874) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
FIH-1/HIF-1AN in C2C12 Mouse Cell Line.
FIH-1/HIF-1AN was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse FIH-1/HIF-1AN Monoclonal Antibody (Catalog # MAB7874) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse FIH-1/HIF-1AN Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line and C2C12 mouse myoblast cell line
Sample: Immersion fixed A172 human glioblastoma cell line and C2C12 mouse myoblast cell line
Simple Western
12.5 µg/mL
Sample: C2C12 mouse myoblast cell line
Sample: C2C12 mouse myoblast cell line
Western Blot
0.25 µg/mL
Sample: A172 human glioblastoma cell line and C2C12 mouse myoblast cell line
Sample: A172 human glioblastoma cell line and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FIH-1/HIF-1AN
Long Name
Factor Inhibiting HIF-1
Alternate Names
FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase
Gene Symbol
HIF1AN
UniProt
Additional FIH-1/HIF-1AN Products
Product Documents for Human/Mouse FIH-1/HIF-1AN Antibody
Product Specific Notices for Human/Mouse FIH-1/HIF-1AN Antibody
For research use only
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