Human/Mouse Glutaredoxin 1/GLRX1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3399
Key Product Details
Species Reactivity
Human, Mouse
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GLRX1
Met1-Gln106
Accession # P35754
Met1-Gln106
Accession # P35754
Specificity
Detects human and mouse GLRX1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Glutaredoxin 1/GLRX1 Antibody
Detection of Human/Mouse Glutaredoxin 1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Glutaredoxin 1/GLRX1 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glutaredoxin 1/GLRX1 at approximately 10 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Glutaredoxin 1/GLRX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3399) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Glutaredoxin 1/GLRX1 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line
Reviewed Applications
Read 4 reviews rated 4.3 using AF3399 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutaredoxin 1/GLRX1
Alternate Names
GLRX, Thioltransferase
Gene Symbol
GLRX
UniProt
Additional Glutaredoxin 1/GLRX1 Products
Product Documents for Human/Mouse Glutaredoxin 1/GLRX1 Antibody
Product Specific Notices for Human/Mouse Glutaredoxin 1/GLRX1 Antibody
For research use only
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