Human/Mouse HSF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5227
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), RNA immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HSF2
Ser411-Ser536
Accession # Q03933
Ser411-Ser536
Accession # Q03933
Specificity
Detects endogenous human and mouse HSF2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse HSF2 Antibody
Detection of Human/Mouse HSF2 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSF2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse HSF2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSF2
HSF2 (Heat shock transcription factor 2; also HSTF2) is a 60 kDa member of the HSF family of proteins. It is widely expressed and serves as a transcriptional activator of HSPs. In quiescent cells, HSF2 is a cytoplasmic homodimer; upon exposure to stress or differentiation, it homo- or hetero-trimerizes with HSF-1, and translocates to the nucleus where it impacts gene transcription. Human HSF2 is 536 amino acids (aa) in length and contains a DNA binding region (aa 7‑107), two NLSs (aa 108‑122 and 195‑210) and an HR (hydrophobic repeat) domain that mediates trimerization (aa 119‑192). There are three potential splice variants. One shows a Leu substitution for aa 260‑536, a second shows an IlePhe substitution for aa 229‑536, and a third shows a deletion of aa 393‑410. Over aa 411‑536, human HSF2 is 92% aa identical to mouse HSF2.
Long Name
Heat Shock Transcription Factor 2
Alternate Names
HSTF2
Entrez Gene IDs
3298 (Human)
Gene Symbol
HSF2
UniProt
Additional HSF2 Products
Product Documents for Human/Mouse HSF2 Antibody
Product Specific Notices for Human/Mouse HSF2 Antibody
For research use only
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