Human/Mouse IL-19 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2915

R&D Systems, part of Bio-Techne
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MAB2915
MAB2915-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 350114

View all IL-19 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-19
Leu25-Ala176
Accession # Q8CJ70

Specificity

Detects human and mouse IL-19 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human/Mouse IL-19 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IL-19 (Catalog # 1035-IL)
Recombinant Mouse IL-19 (Catalog # 2915-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha-helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2 - 4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). It is secreted as a glycosylated monomer, 35 - 45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity with rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL‑19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

References

  1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
  4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
  5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
  6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
  7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
  8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
  13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.

Long Name

Interleukin 19

Alternate Names

IL19

Entrez Gene IDs

29949 (Human); 329244 (Mouse)

Gene Symbol

IL19

UniProt

Q8CJ70

Additional IL-19 Products

Product Documents for Human/Mouse IL-19 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse IL-19 Antibody

For research use only

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