Human/Mouse LRRTM2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5589
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRTM2
Cys34-Arg422
Accession # O43300
Cys34-Arg422
Accession # O43300
Specificity
Detects human and mouse LRRTM2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) LRRTM1, rhLRRTM3, and rhLRRTM4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse LRRTM2 Antibody
LRRTM2 in Embryonic Mouse Neural Tube.
LRRTM2 was detected in immersion fixed frozen sections of embryonic mouse neural tube (E10.5) using 10 µg/mL Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human/Mouse LRRTM2 by Western Blot.
Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
LRRTM2 in Mouse Embryo.
LRRTM2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse LRRTM2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse neural tube (E10.5)
Sample: Immersion fixed frozen sections of embryonic mouse neural tube (E10.5)
Western Blot
1 µg/mL
Sample: Human brain cortex and mouse brain tissue
Sample: Human brain cortex and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRTM2
Long Name
Leucine Rich Repeat Transmembrane Neuronal 2
Alternate Names
LRRN2
Gene Symbol
LRRTM2
UniProt
Additional LRRTM2 Products
Product Documents for Human/Mouse LRRTM2 Antibody
Product Specific Notices for Human/Mouse LRRTM2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...