Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 143908

View all MMP-24/MT5-MMP Antibodies »

Product Summary for Human/Mouse MMP-24/MT5-MMP Antibody

Immunogen

E. coli-derived recombinant mouse MMP‑24/MT5-MMP
Arg125-Gln296
Accession # Q9R0S2

Specificity

Detects mouse MMP‑24/MT5-MMP in direct ELISAs and detects human and mouse MMP‑24/MT5-MMP in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-9, and the catalytic domains of recombinant human MMP‑14, 15, and 16 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse MMP-24/MT5-MMP Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A172 human glioblastoma cell line and bEnd.3 mouse endothelioma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human/Mouse MMP‑24/MT5-MMP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-24/MT5-MMP

MMP-24, also known as MT5-MMP, is a 62-65 kDa member of the M10 metallopeptidase family, a subfamily of MMPs. It is a type I transmembrane glycoprotein that is converted into a 58 kDa active form (amino acids [aa] 129-618) following removal of its propeptide. Proteolytic cleavage after Arg554 generates a 52 kDa active, soluble form that undergoes further cleavage in to 27-34 kDa fragment. MMP-24 has restricted expression, being found on neurons, mast cells, neural stem cells and ependymal cells lining the ventricles of the brain. It is known to cleave chondroitin sulfate, dermatan sulfate, pro-MMP-2, and N-Cadherin. Its activation of MMP-2 likely contributes to neurite outgrowth, while its cleavage of N-Cadherin in-trans between neural stem cells and ependymal initiates stem cell expansion and proliferation. Over amino acids 125-296, mouse MMP-24 shares 100% and 99% aa sequence identity with rat and human MMP-24, respectively.

Long Name

Matrix Metalloproteinase 24/Membrane Type 5 MMP

Alternate Names

MMP24, MT5-MMP

Entrez Gene IDs

10893 (Human); 17391 (Mouse)

Gene Symbol

MMP24

UniProt

Q9R0S2

Additional MMP-24/MT5-MMP Products

Product Documents for Human/Mouse MMP-24/MT5-MMP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse MMP-24/MT5-MMP Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.