Human/Mouse MYB Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6209
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MYB
Lys503-Met640
Accession # P10242
Lys503-Met640
Accession # P10242
Specificity
Detects human and mouse MYB in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse MYB Antibody
Detection of Human and Mouse MYB by Western Blot.
Western blot shows lysates of CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse MYB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MYB at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse MYB Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH‑1 mouse B cell lymphoma cell line
Sample: CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH‑1 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MYB
MYB (myeloblastosis protooncogene) is an 80 kDa transcriptional activator. It is found in hematopoietic and intestinal crypt epithelial cells. MYB interacts with p300 and activates multiple genes, including Bcl2, GATA3, and MYC. Although MYB is thought of as a pro-proliferation and antidifferentiation factor, it would now appear that MYB contributes to both T- and B-cell maturation and to cell survival in general. Human MYB is 640 amino acids (aa) in length. It contains three DNA binding, HTH‑myb type domains (aa 35‑193), one transcriptional activation segment (aa 275‑327), and a Leu‑zipper motif (aa 376‑397). There are multiple potential splice forms. One shows an alternate start site at Met189 coupled with a 99 aa substitution for aa 567‑640. Another shows a 121 aa insertion after Ser401. Four show C‑terminal substitutions: a 34 aa substitution for aa 314‑640, a Met substitution for aa 402‑640, a seven aa substitution for aa 317‑640, and a 10 aa substitution for aa 603‑640. Over aa 503‑640, human MYB shares 84% aa identity with mouse MYB.
Long Name
v-myb Myeloblastosis Viral Oncogene Homolog
Alternate Names
Cmyb;c-myb;c-myb_CDS;efg;Transcriptional activator Myb
Gene Symbol
MYB
UniProt
Additional MYB Products
Product Documents for Human/Mouse MYB Antibody
Product Specific Notices for Human/Mouse MYB Antibody
For research use only
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