MYF-5 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MYF-5 Monoclonal Antibody (Catalog # MAB4027) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of MYF-5 in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse MYF-5 Monoclonal Antibody (Catalog # MAB4027, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human/Mouse MYF-5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: C2C12 mouse myoblast cell line fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 1 review rated 5 using MAB4027 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYF-5

MYF-5 (Myogenic Factor 5), also known as bHLHc2, is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.

Long Name

Myogenic Regulatory Factor 5

Alternate Names

bHLHc2, MYF5

Entrez Gene IDs

4617 (Human); 17877 (Mouse); 299766 (Rat)

Gene Symbol

MYF5

UniProt

P13349 (Human)

Additional MYF-5 Products

Product Documents for Human/Mouse MYF-5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse MYF-5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse MYF-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4027

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MYF-5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse MYF-5 Antibody

MYF‑5 in C2C12 Mouse Cell Line.