Human/Mouse NCOA3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5076
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NCOA3
Met673-Val856
Accession # Q9Y6Q9
Met673-Val856
Accession # Q9Y6Q9
Specificity
Detects endogenous human and mouse NCOA3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse NCOA3 Antibody
Detection of Human NCOA3 by Western Blot.
Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse NCOA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NCOA3 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse NCOA3 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NCOA3
Nuclear Receptor Coactivator 3 (NCOA3) functions in the multisubunit coactivator complex. Multiple nuclear hormone receptors are able to bind to NCOA3 in a hormone dependent manor. NCOA3 contains intrinsic histone acetyltransferase activity, but is also able to recruit other histone aceytltransferases (CBP, PCAF) and methyltransferases (PRMT1, CARM1) to active promoters. NCOA3 null mice exhibit hematological, adiopogenesis, and endotoxic impairments. mRNA of NCOA3 is often amplified in several cancers including breast and ovarian.
Long Name
Nuclear Receptor Co-activator 3
Alternate Names
ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1
Gene Symbol
NCOA3
UniProt
Additional NCOA3 Products
Product Documents for Human/Mouse NCOA3 Antibody
Product Specific Notices for Human/Mouse NCOA3 Antibody
For research use only
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