What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/NALP3 APC-conjugated Monoclonal Antibody (Catalog # IC7578A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of NLRP3/NALP3 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse NLRP3/NALP3 APC-conjugated Monoclonal Antibody (Catalog # IC7578A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line and human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using IC7578A in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NLRP3/NALP3

NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1

Entrez Gene IDs

114548 (Human); 216799 (Mouse); 287362 (Rat)

Gene Symbol

NLRP3

UniProt

Q8R4B8

Additional NLRP3/NALP3 Products

Product Documents for Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

For research use only

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Flow Cytometry

Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC7578A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all NLRP3/NALP3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse NLRP3/NALP3 APC-conjugated Antibody

Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.