Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2156
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Nurr1/NGFI‑B beta/NR4A2
Val332-Lys558
Accession # Q06219
Val332-Lys558
Accession # Q06219
Specificity
Detects human and mouse Nurr1/NGFI‑B beta/NR4A2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antibody
Nurr1/NGFI-B beta/NR4A2 in HeLa Human Cell Line.
Nurr1/NGFI-B beta/NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Nurr1/NGFI-B beta/NR4A2 in Mouse Brain (Hippocampus).
Nurr1/NGFI-B beta/NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of mouse brain (hippocampus)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Nurr1/NGFI‑B beta/NR4A2
Sample: Recombinant Mouse Nurr1/NGFI‑B beta/NR4A2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nurr1/NGFI-B beta/NR4A2
Long Name
Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2
Alternate Names
NGFI-B beta, NOT, NR4A2, TINUR
Gene Symbol
NR4A2
UniProt
Additional Nurr1/NGFI-B beta/NR4A2 Products
Product Documents for Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antibody
Product Specific Notices for Human/Mouse Nurr1/NGFI-B beta/NR4A2 Antibody
For research use only
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