Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM763
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 97505
Product Specifications
Immunogen
KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612
Accession # O43612
Specificity
Detect human and mouse Orexin A/Hypocretin‑1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody
Detection of Orexin A/Hypocretin-1 in Human Hypothalamus.
Orexin A/Hypocretin-1 was detected in immersion fixed paraffin-embedded sections of Human Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin-1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Orexin A/Hypocretin-1 in Mouse Hypothalamus.
Orexin A/Hypocretin-1 was detected in perfusion fixed frozen sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin-1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hypothalamus and perfusion fixed frozen sections of Mouse Hypothalamus
Sample: Immersion fixed paraffin-embedded sections of Human Hypothalamus and perfusion fixed frozen sections of Mouse Hypothalamus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Orexin A/Hypocretin-1
References
- Sakurai, T. et al. (1998) Cell 92:573.
Alternate Names
HCRT, Hcrt1, Hypocretin-1, Hypocretin1
Gene Symbol
HCRT
UniProt
Additional Orexin A/Hypocretin-1 Products
Product Documents for Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody
Product Specific Notices for Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody
For research use only
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