betaTC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of PDX‑1/IPF1 in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells by Flow Cytometry.

BG01V human embryonic stem cells either (A) untreated or (B) differentiated into pancreatic progenitor cells were stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: betaTC‑6 mouse beta cell insulinoma cell line or BG01V human embryonic stem cells differentiated into pancreatic progenitor cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDX-1/IPF1

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Entrez Gene IDs

3651 (Human); 18609 (Mouse)

Gene Symbol

PDX1

UniProt

P52945

Additional PDX-1/IPF1 Products

Product Documents for Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2419P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all PDX-1/IPF1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

Detection of PDX‑1/IPF1 in betaTC‑6 Mouse Cell Line by Flow Cytometry.