Human/Mouse PKC beta2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB43781
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 469827
Product Specifications
Immunogen
E. coli-derived recombinant human PKC beta2
Lys607-Ser673
Accession # P05771
Lys607-Ser673
Accession # P05771
Specificity
Detects human PKC beta2 in direct ELISAs. Detects human and mouse PKC beta2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse PKC beta2 Antibody
Detection of Human and Mouse PKC beta2 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CEM human T-lymphoblastoid cell line, and TK-1 mouse T cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKC beta2 Monoclonal Antibody (Catalog # MAB43781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC beta2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKC beta2 in Human Spleen.
PKC beta2 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human/Mouse PKC beta2 Monoclonal Antibody (Catalog # MAB43781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse/Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse PKC beta2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CEM human T-lymphoblastoid cell line, and TK‑1 mouse T cell lymphoma cell line
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CEM human T-lymphoblastoid cell line, and TK‑1 mouse T cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC beta 2
Long Name
Protein Kinase C beta 2
Alternate Names
PKCB;PKCbeta;PKC-beta;PKCI(2);PRKCB;PRKCB1;PRKCB2;Protein kinase C beta type
Gene Symbol
PRKCB
UniProt
Additional PKC beta 2 Products
Product Documents for Human/Mouse PKC beta2 Antibody
Product Specific Notices for Human/Mouse PKC beta2 Antibody
For research use only
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