Human/Mouse Plexin C1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3887
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse, Bovine
Applications
Validated:
Immunohistochemistry
Cited:
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 544232
Product Specifications
Immunogen
E. coli-derived recombinant human Plexin C1
Ile49-Ser161
Accession # O60486
Ile49-Ser161
Accession # O60486
Specificity
Detects human Plexin C1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no
cross-reactivity with recombinant human Plexin A4, B1, B2, B3, D1, recombinant
mouse Plexin A1, A2, or A3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Plexin C1 Antibody
Plexin C1 in Human Melanoma.
Plexin C1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Plexin C1 in Mouse Embryo.
Plexin C1 was detected in immersion fixed frozen sections of mouse embryonic gut (12 d.p.c.) using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse Plexin C1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma and immersion fixed frozen sections of mouse embryonic gut (12 d.p.c.)
Sample: Immersion fixed paraffin-embedded sections of human melanoma and immersion fixed frozen sections of mouse embryonic gut (12 d.p.c.)
Reviewed Applications
Read 1 review rated 5 using MAB3887 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin C1
Alternate Names
CD232, PLXNC1, VESPR
Gene Symbol
PLXNC1
UniProt
Additional Plexin C1 Products
Product Documents for Human/Mouse Plexin C1 Antibody
Product Specific Notices for Human/Mouse Plexin C1 Antibody
For research use only
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