Human/Mouse PVRIG Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9365
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 969102
Product Specifications
Immunogen
Human PVRIG synthtic peptide
Accession # Q6DKI7
Accession # Q6DKI7
Specificity
Detects human and mouse PVRIG in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse PVRIG Antibody
Detection of Human and Mouse PVRIG by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and TK-1 mouse T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PVRIG at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PVRIG in MOLT‑4 Human Cell Line.
PVRIG was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse PVRIG Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and TK‑1 mouse T cell lymphoma cell line
Sample: Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and TK‑1 mouse T cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PVRIG
References
- Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.
Long Name
Poliovirus Receptor Related Immunoglobulin Domain Containing
Alternate Names
C7orf15, CD112R
Gene Symbol
PVRIG
UniProt
Additional PVRIG Products
Product Documents for Human/Mouse PVRIG Antibody
Product Specific Notices for Human/Mouse PVRIG Antibody
For research use only
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