Human/Mouse Pygopus-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3617
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 466023
Product Specifications
Immunogen
E. coli-derived recombinant human Pygopus‑1
Ala2-Ala419
Accession # Q9Y3Y4
Ala2-Ala419
Accession # Q9Y3Y4
Specificity
Detects human and mouse Pygopus-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Pygopus-1 Antibody
Detection of Human and Mouse Pygopus‑1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Pygopus-1 Mono-clonal Antibody (Catalog # MAB3617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pygopus-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse Pygopus-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using MAB3617 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pygopus-1
Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta-catenin in the nucleus during Wnt-signaling. In particular, following beta-catenin activation and translocation to the nucleus, beta-catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta-catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.
Alternate Names
Pygo1, Pygopus1
Gene Symbol
PYGO1
UniProt
Additional Pygopus-1 Products
Product Documents for Human/Mouse Pygopus-1 Antibody
Product Specific Notices for Human/Mouse Pygopus-1 Antibody
For research use only
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