Human/Mouse Rab7a Antibody

Name: Human/Mouse Rab7a Antibody
Brand: R&D Systems
SKU: AF7789

R&D Systems, part of Bio-Techne | Catalog # AF7789

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Rab7a Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rab7a
Thr2-Ser204
Accession # P51149

Specificity

Detects human and mouse Rab7a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Rab9a and rhRab9b is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Rab7a Antibody

Detection of Human and Mouse Rab7a by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Rab7a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7789) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rab7a at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Rab7a by Simple Western^TM.

Simple Western shows lysates of SK-Mel-28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Rab7a at approximately 29 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse Rab7a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7789). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Applications for Human/Mouse Rab7a Antibody

Application

Recommended Usage

Simple Western

50 µg/mL
Sample: SK-Mel-28 human malignant melanoma cell line

Western Blot

1 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line and B16‑F1 mouse melanoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rab7a

Rab7a (Ras-related protein Rab 7A; also RAB7) is a 22-24 kDa member of the Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as skeletal muscle, neurons, osteoclasts and macrophages. Rab7a plays a key role in the formation of phagolysosomes. In particular, Rab7a-GTP becomes associated with early phagosomes. The GTP form of Rab7a first recruits RILP, a molecule that interacts with ORPL1 and promotes the formation of a dynactin:dynein complex. This complex now initiates the movement of the phagosome along microtubules, where it fuses with lysosomes and endosomes at select points, creating phagolysosomes. Human Rab7a is 207 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C‑terminal CXC prenylation sequence (aa 205-207). There is one utilized phosphorylation site at Tyr183. Full-length human and mouse Rab7a have identical aa sequences. Notably, however, point mutations with single aa changes are noted in both species. By contrast, human Rab7a and Rab7b share less than 50% aa sequence identity, each representing the product of distinct genes.

Long Name

RAs Genes from Brain Protein 7a

Alternate Names

PRO2706, RAB7

Entrez Gene IDs

7879 (Human); 19349 (Mouse); 29448 (Rat)

Gene Symbol

RAB7A

UniProt

P51149 (Human)

Additional Rab7a Products

Product Documents for Human/Mouse Rab7a Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse Rab7a Antibody

For research use only

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