Human/Mouse/Rat 14-3-3 sigma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4424
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human 14-3-3 sigma
Met1-Ser248
Accession # P31947
Met1-Ser248
Accession # P31947
Specificity
Detects human, mouse, and rat 14-3-3 sigma in Western blots. Does not cross react with other recombinant human 14‑3‑3 isoforms.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat 14-3-3 sigma Antibody
Detection of Human/Mouse/ Rat 14‑3‑3 sigma by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3s Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 sigma was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human 14‑3‑3 sigma Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 14-3-3s knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat 14-3-3s Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 14-3-3s at approximately 28 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat 14-3-3 sigma Antibody
Application
Recommended Usage
Knockout Validated
14‑3‑3 sigma
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
14‑3‑3 sigma knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line and mouse brain tissue
Sample: A431 human epithelial carcinoma cell line and mouse brain tissue
Reviewed Applications
Read 1 review rated 5 using AF4424 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 14-3-3 sigma
14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta.
Alternate Names
1433 sigma, Epithelial cell marker protein 1, SFN, YWHAS
Entrez Gene IDs
2810 (Human)
Gene Symbol
SFN
UniProt
Additional 14-3-3 sigma Products
Product Documents for Human/Mouse/Rat 14-3-3 sigma Antibody
Product Specific Notices for Human/Mouse/Rat 14-3-3 sigma Antibody
For research use only
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