Human/Mouse/Rat alpha-Cardiac Actin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9308
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 959606
Product Summary for Human/Mouse/Rat alpha-Cardiac Actin Antibody
Immunogen
E.coli-derived recombinant human alpha-Cardiac Actin
Asp3-Phe377
Accession # P68032
Asp3-Phe377
Accession # P68032
Specificity
Detects human alpha-Cardiac Actin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat alpha-Cardiac Actin Antibody
Detection of Human, Mouse, and Rat alpha-Cardiac Actin by Western Blot.
Western blot shows lysates of human, mouse, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/ Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Cardiac Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha-Cardiac Actin in Human Cardiomyocytes.
a-Cardiac Actin was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated with methanol. Specific staining was localized to cytoplasm.
alpha-Cardiac Actin in Human Heart.
a-Cardiac Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Cardiac Actin Monoclonal Antibody (Catalog # MAB9308) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat alpha-Cardiac Actin Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed human induced pluripotent stem cells (iPSC) differentiated to cardiomyocytes
Sample: Immersion fixed human induced pluripotent stem cells (iPSC) differentiated to cardiomyocytes
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Simple Western
2 µg/mL
Sample: Human, mouse, and rat heart tissue
Sample: Human, mouse, and rat heart tissue
Western Blot
0.1 µg/mL
Sample: Human, mouse, and rat heart tissue
Sample: Human, mouse, and rat heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB9308 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Cardiac Actin
Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.
Long Name
Actin, Alpha, Cardiac Muscle 1
Alternate Names
ACTC, ACTC1, ASD5, Cardiac alpha-Actin, CMD1R, CMH11, LVNC4
Gene Symbol
ACTC1
UniProt
Additional alpha-Cardiac Actin Products
Product Documents for Human/Mouse/Rat alpha-Cardiac Actin Antibody
Product Specific Notices for Human/Mouse/Rat alpha-Cardiac Actin Antibody
For research use only
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