Human/Mouse/Rat alpha-Synuclein Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13381
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 913611
Product Specifications
Immunogen
E. coli-derived recombinant human alpha-Synuclein
Met1-Ala140
Accession # P37840
Met1-Ala140
Accession # P37840
Specificity
Detects human alpha-Synuclein in direct ELISAs and human, mouse and rat alpha-Synuclein in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat alpha-Synuclein Antibody
Detection of Human, Mouse, and Rat alpha-Synuclein by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat a-Synuclein Monoclonal Antibody (Catalog # MAB13381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha-Synuclein in Human Brain.
a-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human/Mouse/Rat a-Synuclein Monoclonal Antibody (Catalog # MAB13381) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic varicosities. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat alpha-Synuclein Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra)
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra)
Western Blot
2 µg/mL
Sample: Human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Synuclein
Alternate Names
NACP, PARK1, PARK4, SNCA, Synuclein-alpha
Gene Symbol
SNCA
UniProt
Additional alpha-Synuclein Products
Product Documents for Human/Mouse/Rat alpha-Synuclein Antibody
Product Specific Notices for Human/Mouse/Rat alpha-Synuclein Antibody
For research use only
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