Human/Mouse/Rat Annexin A1 Antibody

Detection of Human/Mouse/Rat Annexin A1 by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Annexin A1 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human and Mouse Annexin A1 by Simple Western^TM.

Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (U87 MG) (NBP2-49844) and MOLT-4 human acute lymphoblastic leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A1 at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Annexin A1/Annexin I in Human Breast.

Annexin A1/Annexin I was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse/Rat Annexin A1/Annexin I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.