Human/Mouse/Rat BTF3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5747
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BTF3
Lys46-Asn206
Accession # P20290
Lys46-Asn206
Accession # P20290
Specificity
Detects human, mouse and rat BTF3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat BTF3 Antibody
Detection of Human/Mouse/Rat BTF3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SK-BR-3 human breast cancer cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat BTF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5747) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BTF3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BTF3 in Human Pancreas Cancer Tissue.
BTF3 was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Human/Mouse/Rat BTF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5747) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat BTF3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreas cancer tissue
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, SK-BR-3 human breast cancer cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line
Sample: HeLa human cervical epithelial carcinoma cell line, SK-BR-3 human breast cancer cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTF3
Long Name
Basic Transcription Factor 3
Alternate Names
BETA-NAC, NACB
Gene Symbol
BTF3
UniProt
Additional BTF3 Products
Product Documents for Human/Mouse/Rat BTF3 Antibody
Product Specific Notices for Human/Mouse/Rat BTF3 Antibody
For research use only
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