Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2185
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase III/CA3
Ala2-Lys260
Accession # P07451
Ala2-Lys260
Accession # P07451
Specificity
Detects human Carbonic Anhydrase III/CA3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
Detection of Human, Mouse, and Rat Carbonic Anhydrase III/CA3 by Western Blot.
Western blot shows lysates of human, mouse, and rat skeletal muscle tissue and heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Carbonic Anhydrase III/CA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2185) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Carbonic Anhydrase III/CA3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase III (Catalog # 2185-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase III (Catalog # 2185-CA), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Human, mouse, and rat skeletal muscle tissue and heart tissue
Sample: Human, mouse, and rat skeletal muscle tissue and heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase III/CA3
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).
Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
Alternate Names
CA3
Entrez Gene IDs
761 (Human)
Gene Symbol
CA3
UniProt
Additional Carbonic Anhydrase III/CA3 Products
Product Documents for Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
Product Specific Notices for Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
For research use only
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