Human/Mouse/Rat CARD11/CARMA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4839
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CARD11
Lys263-Ser442
Accession # Q9BXL7
Lys263-Ser442
Accession # Q9BXL7
Specificity
Detects endogenous human, mouse, and rat CARMA1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat CARD11/CARMA1 Antibody
Detection of Human/Mouse/Rat CARD11/CARMA1 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CARD11/CARMA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4839) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CARD11/CARMA1 at approximately 133 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat CARD11/CARMA1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line
Sample: Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CARD11/CARMA1
CARMA1 (CARD-MAGUK1; also CARD11) is a 133 kDa member of the MAGUK family of proteins. It is a cytoplasmic promoter of Bcl10 phosphorylation, and as such, regulates the effectiveness of NF-kappa B signaling. Human CARMA1 is 1154 amino acids (aa) in length and contains an N-terminal CARD region, two coiled-coil (CC) domains (aa 123‑250 and 295‑442), and a PZD, SH3 and MAGUK domain (aa 996‑1133). The CC1 and MAGUK domains position the molecule, while CC1 and CC2 mediate a necessary dimerization/oligomerization. There is one potential alternate start site seven aa upstream from the standard start site. Over aa 263-442, human CARMA1 is 98% aa identical to both mouse and canine CARMA1.
Long Name
Caspase Recruitment Domain-containing Protein 11
Alternate Names
BIMP3, CARMA1
Gene Symbol
CARD11
UniProt
Additional CARD11/CARMA1 Products
Product Documents for Human/Mouse/Rat CARD11/CARMA1 Antibody
Product Specific Notices for Human/Mouse/Rat CARD11/CARMA1 Antibody
For research use only
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