Human/Mouse/Rat CART Antibody

R&D Systems, part of Bio-Techne | Catalog # AF163

R&D Systems, part of Bio-Techne
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AF163
AF163-SP
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Conjugate
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Biotin
BAF163
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CART Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CART
Gln28-Leu116
Accession # Q16568

Specificity

Detects human CART in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69-116.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat CART Antibody

CART antibody in Human Brain Hippocampus Tissue by Immunohistochemistry (IHC-P).

Detection of CART in Human Brain Hypothalamus Tissue.

CART was detected in immersion fixed paraffin-embedded sections of human brain (Hypothalamus) tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.
CART antibody in Mouse Brain Tissue by Immunohistochemistry (IHC-Fr).

CART in Mouse Brain Tissue.

CART was detected in perfusion fixed frozen sections of mouse brain tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. Staining was performed using our Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat CART Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (Hypothalamus) tissue and perfusion fixed frozen sections of mouse brain tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human CART
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CART

CART, also known as cocaine and amphetamine-regulated transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations. In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment. There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region. There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist. These include a 5K, 48 aa peptide (CART 42-89), and a 4.3K, 40 aa peptide (CART 49-89). Human CART is 98% aa identical to both moyuse and rat CART (1-89). Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions. In addition, anatomical location is associated with differing lengths of CART. For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

References

  1. Douglass, J. & S. Daoud (1996) Gene 169:241.
  2. Douglass, J. et al. (1995) J. Neurosci. 15:247.
  3. Adams, L.D. et al. (1999) Brain Res. 848:137.
  4. Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
  5. Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
  6. Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
  7. Thim, L. et al. (1998) FEBS Lett. 428:263.

Long Name

Cocaine- and Amphetamine-regulated Transcript Protein

Alternate Names

CARTPT

Entrez Gene IDs

9607 (Human)

Gene Symbol

CARTPT

UniProt

Q16568

Additional CART Products

Product Documents for Human/Mouse/Rat CART Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CART Antibody

For research use only

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